柠檬醛对指状青霉糖酵解的影响

Effects of Citral on Glycolysis of Penicillium digitatum

本研究测定了不同浓度柠檬醛对指状青霉呼吸代谢及糖酵解途径相关指标的影响。结果表明,柠檬醛能明显抑制指状青霉的呼吸代谢,1.0μL/m L浓度下柠檬醛与糖酵解途径、三羧酸循环途径以及磷酸戊糖途径典型抑制剂(碘乙酸,丙二酸,磷酸钠)的叠加率分别为15.00%、12.60%和30.70%,且此结果显示糖酵解途径受到了柠檬醛的影响。经最小抑菌浓度(minimal inhibitory concentration,MIC,2.0μL/m L)和最小杀菌浓度(minimal fungicidal concentration,MFC,4.0μL/m L)柠檬醛处理后,指状青霉糖酵解途径重要的中间产物葡萄糖和丙酮酸的含量显著增加,己糖激酶(Hexokinase,HK)的活性下降,6-磷酸果糖激酶(Phosphofructokinase,PFK)和丙酮酸激酶(Pyruvate kinase,PK)的活性增加。本研究表明柠檬醛能够抑制指状青霉的呼吸代谢,改变指状青霉糖酵解途径关键酶的活性和重要中间产物的含量。

The effects of citral on the respiratory metabolism and glycolysis of Penicillium digitatum were determined. Results showed that citral has a significant inhibitory effect on the respiratory metabolism of P. digitatum and the superposing percentages of treatment with 1.0 μL/mL citral after addition of malonic acid, iodine acetic acid, and phosphate sodium were 15.00%, 12.60% and 30.70%, respectively. Determination of the superposition percent demonstrated that the glycolytic pathway was affected by citral. After treatment with citral at its minimal inhibitory concentration (MIC, 2.0 μL/mL) and minimal fungicidal concentration (MFC, 4.0 μL/mL), marked increases in theglucose content, pyruvic acid content, phosphofructokinase activity, and pyruvate kinase activity were observed. Moreover, a reduction in hexokinase activity was also observed. The results of this study indicate that citral affects the activity of key enzymes and the content of important intermediate products of the glycolytic pathway of Penicillium digitatum. © 2015, South China University of Technology. All right reserved.