L-阿拉伯糖促进秀丽隐杆线虫的生长发育及降低脂肪合成

L-arabinose Promotes the Growth and Development of Caenorhabditis elegans and Reduces Fat Synthesis

本文以秀丽隐杆线虫作为模式生物,研究L-阿拉伯糖对其生长发育及脂肪合成能力的影响。试验采用葡萄糖作为对照组,分别添加浓度为2.5、5、7.5、10、20mmol/L的L-阿拉伯糖作为试验组,将线虫进行同步化处理后,分析线虫头部摆动频率、身体弯曲频率、身体长度、子代数目及其甘油三酯含量和油红O染色结果。结果显示,在现有添加浓度下,L-阿拉伯糖对秀丽隐杆线虫的身体弯曲频率、子代数目无显著影响,但对线虫的头部摆动频率、身体长度影响显著:当浓度为20 mmol/L时,线虫头部摆动频率相对于对照组增加了约20%;当浓度为7.5mmol/L时,线虫身体长度与对照组相比增加了约11%;甘油三酯分析结果显示,L-阿拉伯糖试验组中线虫体内的甘油三脂含量均低于对照组,并随添加浓度的增加显著降低(p<0.01),当L-阿拉伯糖浓度为20 mmol/L时,线虫体内甘油三酯含量与对照组相比降低了约67%;油红O染色结果与甘油三酯分析结果一致。本试验研究表明,L-阿拉伯糖能够促进秀丽隐杆线虫的头部摆动频率和身体生长,并对于降低其脂肪合成作用具有较为明显的效果,具有一定的降脂功效。

In this work,the effect of L-arabinose on the growth development and fat synthesis capacity of Caenorhabditis elegans was investigated.Glucose was used as control sample while 2.5,5.0,10.0 and 20.0 mmol/L of L-arabinose were added respectively as treatment groups.After pretreatment of synchronized,the head swing frequency,body bending frequency,body length,number of offspring,triglyceride content and oil red O staining of elegans were analyzed.The results showed that L-arabinose had no significant effect on the body bending frequency and number of offspring of Caenorhabdits elegans.However,it had a significant efficiency on the head swinging frequency and body growth of elegans.When the concentration was 20 mmol/L,the head swining frequency increased by 20%.When the concentration of L-arabinose was 7.5 mmol/L,the body length of elegans increased by 11%.The triglyceride analysis revealed that the contents of triglyceride in treatment groups were lower than that of control group and decreased gradually with increasing L-arabinose concentrations.When the concentration was 20 mmol/L,the triglyceride content in elegans reduced by nearly 67%compared with the control group.The results of oil red O staining were consistent with the analysis of triglyceride.Results indicated that L-arabinose can promote the head swining frequency and body growth of Caenorhabdits elegans and has obvious effect on reducing fat synthesis of them,thus has a certain lipid-lowering function.