HPLC测定长柄扁桃仁及饼粕中苦杏仁苷与野黑樱苷

Study on Amygdalin and Prunasin of Amygdalus pedunculata Pall Kernel and Meal by HPLC

为确保长柄扁桃仁在加工与其副产物利用过程中,更快更有效地明确降解产物中有害物质含量,本文建立了一种同时测定样品中苦杏仁苷与其降解产物野黑樱苷的高效液相色谱方法。在采用甲醇提取长柄扁桃仁、饼粕中苦杏仁苷和野黑樱苷,色谱柱Aglient ZORBAX SB-C18(5μm,4.6 mm×250 mm),流动相为20%甲醇和80%水,流速1.00 mL/min,检测波长210 nm,柱温30℃的条件下,苦杏仁苷和野黑樱苷色谱峰分离良好;苦杏仁苷浓度在0.50～300μg/m L间线性关系良好,R2=0.9999,野黑樱苷浓度在0.30～100μg/m L间线性关系良好,R2=0.9999;长柄扁桃仁提取苦杏仁苷和野黑樱苷平均加标回收率分别为87.10%～96.19%(RSD为4.64%～7.83%)和88.65%～103.10%(RSD为3.03%～7.55%);而在长柄扁桃饼粕中分别为98.32%～107.99%(RSD为1.44%～3.36%)和107.58%～117.60%(RSD为1.45%～2.26%)。本方法准确、可靠,适用于长柄扁桃仁、长柄扁桃饼粕中苦杏仁苷与野黑樱苷的测定。

To clear hazardous substances from degradation products more quickly and effectively in Amygdalus pedunculata Pall meal and its by-products, high performance liquid chromatography(HPLC) was established, the amygdalin and prunasin could be determined simultaneously. When methanol was used to extract amygdalin and prunasin in Amygdalus pedunculata Pall kernel and Amygdalus pedunculata Pall meal, the suitable determination conditions were as follows: Agilent ZORBAX SB-C18 column(5 μm, 4.6 mm×250 mm), mobile phase was 20% methanol and 80% water, the flow rate was 1.00 mL/min, the detection wavelength was 210 nm, column temperature 30 ℃. The peaks of amygdalin and prunasin were isolated well;the linear range for amygdalin was 0.50~300 μg/m L(R2=0.9999), while the prunasin was 0.30~100 μg/m L(R2=0.9999);the average recovery of amygdalin of Amygdalus pedunculata Pall kernel and meal were 87.10%~96.19%(RSD=4.64%~7.83%), 98.32%~107.99%(RSD=1.44%~3.36%), respectively;while the average recovery of prunasin were 88.65%~103.10%(RSD=3.03%~7.55%), 107.58%~117.60%(RSD=1.45%~2.26%), respectively. The method is accurate and reliable, and can be used in the determination of amygdalin and prunasin in Amygdalus pedunculata Pall kernel and meal.