乐昌含笑不同类型鉴定的ISSR-PCR分析

IDENTIFICATION OF MICHELIA TSOI TYPES USING ISSR-PCR MARKER ASSAYS

利用inter -简单重复序列 (ISSR)标记对乐昌含笑 6种类型的遗传变异进行了研究 ,从 30个引物中筛选出 12个多态性引物用于正式扩增 ,共扩增出 134条DNA带 ,其中多态性DNA带 6 7条 ,占 5 0 % ,平均每个引物扩增的DNA带的数目为 11 16 7条。其中引物ISSR16、ISSR19能区分全部类型。引物ISSR16、ISSR19和ISSR2在不同的类型中扩增出了一些特有条带 ,对乐昌含笑类型和品种鉴定以及检验品种的真实性方面非常有价值。本研究对ISSR分析技术的关键步骤进行了讨论 。

Based on introduction and selective breeding of Michelia tsoi , genetic variations of 6 M. tsoi types were determined using ISSR marker assays. 12 primers were screened from 30 primers, and total 134 DNA bands were amplified, 67 of which (50%) were polymorphic. The average number of DNA bands amplified by each primer was 11.167. DNA profiles based on ISSR16, ISSR19 and ISSR2 have revealed potential diagnostic fingerprints and specific bands for various types. The ISSR specific bands are useful for the identification of cultivated germplasm, and testing trueness of cultivars. Key steps of ISSR\|PCR analysis techniques and application to testing trueness and purity of M. tsoi types were discussed. A dendrogram was constructed by using UPGMA algorithm.