富钾植物DNA导入水稻变异后代苗期耐低钾种质的筛选

Screening of K-Deficit Tolerant Offspring from Different Rice Varieties Introduced DNA of K-Sufficiency Plant

为了论证利用分子育种的手段改良水稻的钾素营养特性在技术上的可行性 ,利用测定 K+吸收和 H+释放 ,将富钾植物空心莲子草、商陆 DNA导入到不同水稻品种受体的 3 2个变异后代材料 ,在低钾营养液中进行苗期耐低钾筛选 .结果表明 ,K+吸收和 H+释放速率极显著正相关 ,变异后代的 K+吸收 ,H+释放速率均比其受体显著增加 ,与耐低钾对照材料明恢 63相近 .此外 ,测量这些材料分蘖期的株高、苗干重、根干重 ,验证筛选出的 10个变异后代均为 1级耐低钾材料 。

DNA from K + rich plant Alternathera philoxeroides and Phytolaoca esculenta Van Houtt are introduced into different rice varieties.K + influx and H + efflux of thirty two rice varieties materials has been measured at low K level.The results show,K + influx rate and H + efflux rate of K deficit tolerant rice materials are higher than those of their recipients.Meanwhile,plant height,dry shoot weight and dry root weight of rice are measured at tillering stage at normal K and low K level.Varieties genotype tolerant to K deficiency is greater than their recipients ones.In addition the method improving K nutrition by means of gene engineering is confirmed.