RAPD分子标记技术在茶树亲子鉴定中的应用

Study on the Application of RAPD Technique to Parentage Identification of Tea Plant

为给茶树种质资源的鉴定、品种鉴定、亲子鉴定提供科学方法 ,通过对 3个人工杂交组合的亲本及 F1 代进行 RAPD分析 .结果显示 :双亲的 RAPD谱带能在其 F1 代中表现出来 ,表现率分别为 94.90 % ,97.92 % ,98.64 % ,说明 3个杂交单株是其亲本的真正后代 ,这也表明 RAPD技术在亲子鉴定中具有很强的适用性 .同时从 3个杂交组合的 RAPD分析结果可以看出 ,双亲的 RAPD谱带在 F1 代出现分离现象 。

An analysis of three groups of hybridization combinations by artificial insemination shows that the RAPD bands in parents are nearly found in their offspring,with the appearance rate of 94.90% ,97.92% and 98.64% respectively, which proves that those three tea plants from three hybridization combinations are real hybrid offspring of their parents, and RAPD technique can effectively be used in Parentage identification, cultivars identification and germplasms identification.In addition, it can be found in this study that there are some separate phenomena of RAPD bands among the parents and their offspring. As a result,it can be concluded that every tree plant is a hybrid.