不结球白菜耐热变异体的生长与移栽及品质分析

Growth and Transplanting of Tube-Plantlets Derived from Variants with Higher Heat Tolerance of B.campestris var.Chinensis and the Observation for Their Quality and Field-Characters

以不结球白菜耐热变异体为供试材料 ,比较了不同浓度蔗糖 ,IBA和MET对试管苗生长及发根的影响 ;比较了不同基质 ,炼苗天数和保湿天数对试管苗移栽成活率的影响 ;测定了植株叶片的游离氨基酸含量和观察了植株田间的耐热、耐湿及抗病性。实验结果表明 ,培养基中添加 2 %蔗糖 ,0 1mg/LIBA和 0 1mg/LMET有益于试管苗的健壮及发根 ;用珍珠岩作基质 ,5d炼苗和保湿 10d可以提高移栽成活率 ;耐热变异体中存在氨基酸含量和耐热、耐湿。

The effects of different concentration of sucrose,IBA and MET in medium on growth and rooting of tube-plantlets were compared using variants with higher heat tolerance of B campestris var Chinensis as donor materals The effects of different medium,days for plantlets hardening and days for maintaining humidity on transplanting survival rate of tube-plantlets were also investigated The content of endogenous amino acid within leaf of the variants were tested and the heat-Tolerance,water-logging tolerance and disease resistance of the variants were observed in field The results showed that the vigor and rooting of plantlets were improved by adding 2% sucrose,0 1mg/L IBA and 0 1mg/L MET in medium; the transplanting survival rate of tube-plantlets were able to be increased by using medium of perlit,hardening plantlets for 5 days and maintaining humidity for 10 days Compared with the original variety,the variants with higher content of amino acid,heat tolerance,water-logging tolerance and disease resistance were selected from tested materials