多电极高温射频灭活对兔正常肺组织作用的生物学效应

The biological effects of multi-electrode radiofrequency ablation on normal rabbit lungs

目的 探讨经皮穿刺多电极高温射频术对正常肺组织作用的生物学效应和作用方式。方法 在CT引导下经皮肺穿刺 ,将电极定位于新西兰白兔右肺进行射频消融术 ,并通过CT扫描及光镜、电镜检查观察其生物学效应。结果 射频灭活后即刻 ,镜下可见毁损灶发生凝固性坏死 ,直径为 (1.8± 0 .2 )cm ;第 7天 ,坏死灶开始出现纤维组织增生 ;1个月时支气管上皮和肺泡上皮增生 ,肺泡开始重建 ;2～ 3个月恢复正常的组织结构。电极位于肺门部位治疗时 ,治疗时间、阻抗与电极位于外周部位时比较均有显著性差异 (P <0 .0 1,P <0 .0 1)。结论 多电极高温射频能安全地作用于肺组织 ,并引起一定范围的凝固性坏死。

Objective To study the biological effects of multi electrode radiofrequency ablation on pulmonary tissues of rabbits. Methods Under the guidance of computer tomograph, electrodes were inserted into right lungs of New Zealand white rabbits and radiofrequency was performed. The biological effects were observed through CT image and microscopy. Results Coagulative necrosis was found immediately in ablation area after the procedure. On the 7th postradiofrequency ablation day, fibrous tissues appeared in the necrotic lesions. On the 30th postradiofrequency ablation day, bronchial and alveolar epithelium began to proliferate. Within 60 to 90 days after treatments, the necrotic lesions were almost replaced by normal pulmonary tissues. In group with electrodes into the right hilum, time for treating and initial impedance were significantly different from those with electrodes into the peripheral sites of the right lower lobes (P<0.01, P<0.01). Conclusion Multi electrode radiofrequency ablation can be safely and effectivly performed in pulmonary tissue and cause coagulative necrosis within a certain extent.