摘要
为了建立灵敏度高、操作方便、适用范围较广的测定乙酰胆碱含量的方法，本文采用高效液相色谱加柱后酶衍生化、电化学检测器方法测定了组织中乙酰胆碱的含量。样本处理采用高氯酸沉淀蛋白后一次性高速离心的上清液进样。样品中乙酰胆碱、胆碱和内标可以基线分离，在流速１２ｍｌ·ｍｉｎ－１时，３个峰在１０ｍｉｎ内全部出完。乙酰胆碱的回收率达８５％±２％；样品出峰高度与进样量呈直线关系；重现性好，日内标准偏差为１２％。结果显示本法可以同时测定组织中胆碱及乙酰胆碱的含量，测定灵敏度可达到１ｐｍｏｌ水平。方法简便、快捷。
A rapid and simple method using a combination of high performance liquid chromatography (HPLC), an immobilized enzyme column, with electrochemical detection (ECD) is described for the assay of acetylcholine (ACh) and choline (Ch) in mouse brain. Perchloric acid extracts of small brain tissue were injected onto the HPLC system with no precleanup procedure. Detection limits for both compounds were 1 pmol. The present method has been successfully applied to the measurement of ACh in discrete brain areas of the mouse.
出处
《药学学报》
CAS
CSCD
北大核心
1997年第12期920-923,共4页
Acta Pharmaceutica Sinica
