HPV感染在高发区与低发区宫颈癌中的检测

Detection of HPV Infections in Cervical Carcinoma—In High Incidence Area and Low Incidence Area

收集宫颈癌高发区湖北省五峰县宫颈癌活检组织２５例、宫颈炎２２例，低发区的宫颈癌活检组织４０例、宫颈炎１５例由湖北医科大学附二医院妇瘤科提供。应用ＧＰ－ＰＣＲ（ｇｅｎｅｒａｌｐｒｉｍｅｒｓ－ＰＣＲ）技术检测多型ＨＰＶ感染，发现宫颈癌高发区阳性率为８８．０％（２２／２５），低发区为８７．５％（３５／４０），宫颈炎高发区为３６．４％（８／２２），低发区为６．７％（１／１５）。同时同一份样品进行ＨＰＶ１６型Ｅ７基因的检测，在宫颈癌高发区中检出率为６０．０％（１５／２５），低发区为５２．５％（２１／４０），宫颈炎中为２２．７％（５／２２），而在低发区宫颈炎中没有检测到ＨＰＶ１６Ｅ７基因。结果表明：ＨＰＶ感染在高发区宫颈炎组织中明显高于低发区，两者差异具有显著性。

? The specimens were obtained from 25 cases of cervical carcinoma and 22 cases of cervicitis from Wufeng country, a highincidence area and 40 cases of cervical carcinoma and 15 cases of cervicitis from the Female Tumor Department of Second Affihated Hospital of Hubei Medical University. GPPCR(general primersPCR) has been applied in testing of HPV infection. The positive rates of cervical carcinoma in the high incidence area was 88.0%(22/25) and 87.5%(35/40) in the low incidence area. The rates of cervicitis was 36.4%(8/22) in the high incidence area, and 6.7%(1/15) in the low incidence area. The same specimens were tested with HPV16 E7 gene . The rates of cervical carcinoma was 60.0%(15/25) and cervicitis were 52.5%(21/40) in high incidence area. HPV16 E7 gene wasn’t tested in low incidence area. The results show that HPV infection in cervicitis in high incidence area was higher than in low incidence area (P<0.05). The monitoring of HPV infections is important for prevention and early diagnosis of cervical carcinoma in highrisk population.