端粒酶逆转录酶基因在口腔癌前病变及鳞癌的表达

Telomerase Reverse Transcriptase mRNA Expression in Oral Precancerous Lesion and Squamous Cell Carcinoma

目的 观察端粒酶催化蛋白基因 (h TRT)在口腔癌前病变及鳞癌中的表达 ,探讨其与口腔鳞癌(OSCC)发生发展及临床病理特征之间的关系。 方法 用原位杂交技术检测 5 4例标本 ,其中正常口腔粘膜 6例、上皮非典型增生 15例、口腔粘膜鳞癌 33例。 结果 正常口腔粘膜组织中 h TRT的 m RNA表达较弱 ,阳性信号仅局限于上皮基底层及副基底层间 ,阳性率 16 .6 7% (1/ 6 ) ;上皮非典型增生中 h TRT的 m RNA阳性表达见于多层上皮细胞 ,并随细胞异形性增高而表达增强 ,阳性率 6 0 % (9/ 15 ) ;口腔粘膜鳞癌组织中 h TRT的 m RNA有较强阳性表达 ,阳性率 87.88% (2 9/ 33) ;OSCC组织中 h TRT m RNA的表达与肿瘤临床病理分化无关 ,与淋巴结转移密切相关。 结论 端粒酶 h TRT基因的表达在

Objective\ To investigate the relationship between telomerase human telomerase \{reverse\} transcriptase(hTRT) gene expression and malignant development of oral mucosa epithelium.\ \{Methods\}\ 54 samples were detected by in situ hybridization technique for hTRT gene, including 6 cases of normal oral mucosa, 15 cases of oral precancerous lesions(dysplasia lesion), and 33 cases of oral squamous cell carcinomas.\ Results\ (1)Weaker expression of hTRT mRNA was observed in normal oral mucosa. Positive cells were present in basal layer and parabasal layer, and hTRT positivity was 16 67%(1 of 6).\ (2)The expression of hTRT mRNA in the precancerous lesions was gradually strong associated with phenotypic progression and the degree of dysplasia.\ Positive cell extended to multi\|layered epithelium, and hTRT positive rate was 60%(9 of 15).\ (3)Strong hTRT mRNA expression was observed in squamous cell carcinoma, and the positive rate was 87 88%(29 of 33).\ Conclusion\ Telomerase hTRT gene \{expression\} play a critical role in the occurrence, development and metastasis of oral squamous cell \{carcinomas\}.\;