^(103)Pd对血管平滑肌细胞增殖和凋亡影响的实验研究

Experimental study of the effect of ^( 103)Pd on the proliferation and apoptosis of vascular smooth muscle cells

目的 研究1 0 3Pd对血管平滑肌细胞 (SMC)增殖和凋亡的影响。方法 将不同剂量1 0 3Pd溶液加入SMC培养液中 ,72h时加入1 0 3Pd衰变液作对照 ;应用3H TdR参入实验研究1 0 3Pd对SMC增殖的影响 ,应用流式细胞仪检测1 0 3Pd对SMC凋亡的影响。结果 1 0 3Pd能明显抑制SMC增殖 ,并呈剂量依赖性。 1.85MBq1 0 3Pd对SMC增殖的抑制率为 2 .3% ,几乎不能抑制SMC增殖 ;1 0 3Pd剂量从 7.4MBq增加到 37.0MBq ,增殖抑制率从 4 1.6 %上升至 91.2 % ,SMC增殖受到明显抑制。 1.85～ 37.0MBq1 0 3Pd均不能诱导SMC的明显凋亡 ,其凋亡率均小于 4 .0 %。结论 1 0 3Pd在体外能有效抑制SMC增殖。1 0 3Pd抑制新生内膜形成的机制主要是抑制SMC增殖 ,而非诱导SMC的大量凋亡。

ObjectiveTo investigate the ability of γ emitting radionuclide 103Pd to inhibit the vascular smooth muscle cell (SMC) proliferation and to induce its apoptosis in vitro. Methods 103Pd solution was added to the culture medium to irradiate SMCs for 72 h and non-radioactive Pd solution was added as control. 3 103Pd solution was added to the culture medium to irradiate SMCs for 72 h and non-radioactive Pd solution was added as control. 3H-TdR incorporation test was used to detect the effect of 103Pd on the proliferation of SMCs. Flow cytometer was used to detect the apoptotic SMCs. [WTHResultsThe results showed that inhibition of SMC proliferation was evident and the effects were dose-dependant. Inhibition rate of SMC proliferation by 1.85 MBq 103Pd was 2.3%, which was not significant. The inhibition rate increased from 41.6% to 91.2% as the dose of 103Pd increased from 7.4 to 37.0 MBq, and the proliferation of SMCs was repressed significantly then. The apoptosis rate was extremely low(less than 4.0%) with the 103[KG*4/5]Pd dose escalating from 1.85 to 37.0 MBq. ConclusionsThis study suggests that proliferation of SMCs can be repressed effectively in vitro by 103Pd. 103Pd can be used to inhibit the neointimal proliferation. 103Pd radioactive stent implantation can be employed as a possible novel means to prevent restenosis.