鼠源TRAIL真核表达质粒的构建及其抑瘤效应的初步观察

Construction of Eukaryotic Expressing Plasmid of Mouse TRAIL and Its Inhibitory Effect on Tumor Growth

采用 RT- PCR自小鼠脾细胞 RNA中扩增出鼠 TRAIL 基因的全长 c DNA,利用 DNA重组技术将其插入到真核表达载体 pc DNA3.1中获得重组质粒 p X1。经酶切鉴定及序列分析 ,表明成功构建 TRAIL 的真核表达质粒。通过直接肌肉内注射进行基因转染 ,p X1具有诱导肝癌细胞凋亡、抑制肝癌生长的作用。表明 TRAIL

The mouse TRAIL cDNA was amplified from the RNA of the mouse spleen cells by using RT PCR and cloned into the eukaryotic expressing vector pcDNA3 by DNA recombinant technique to obtain the recombinant plasmid pX1. The eukaryotid expressing plasmid pX1 was constructed successfully which was confirmed by enzyme cleavage identification and sequencing identification. The transfection of plasmid pX1 by injection into muscle of mouse could produce the inhibitory effect on liver carcinoma growth by inducing cells into apoptosis. It was demonstrated that TRAIL could be applied into the gene therapy for liver carcinoma as a direct killing mechanism.