冠心病患者血浆纤维蛋白原β-148C/T基因多态性的研究

Plasma Fibrinogen β-148C/T Polymorphism of Patients with Coronary Heart Disease

为研究β纤维蛋白原基因启动子区 Hind β多态性和血浆纤维蛋白原浓度与冠心病之间的关系。对确诊的冠心病患者 5 6例 ,健康对照组 4 4例 ,采用酚 /氯仿抽提方法从白细胞中提取人基因组 DNA ,经多聚酶链式反应(PCR)加 Hind 内切酶技术检测目的基因片段 ,采用自动化检测系统求出血浆纤维蛋白原 (Fg)浓度。结果发现 :冠心病患者βHind 多态性与血浆纤维蛋白原浓度间存在显著正相关 (r=0 .7,P<0 .0 0 1) ,以非βHind 酶切位点缺失的CT、 CC基因型血浆纤维蛋白原浓度明显增高 (P<0 .0 1)。提示 :β Hind

In order to study the association between plasma fibrinogen levels and the HindⅢ polymorphisms located in the promoter rigion of the β fibrinogen gene in patients with coronary heart disease (CHD), DNA was extracted from white cells of 56 subjects with CHD and 44 controls using the phenol/chloroform method. HindⅢ genotypes were analyzed using polymerase chain reaction combined with HindⅢ (endonuclease) enzyme cutting methods. Plasma fibrinogen was measured by the kenetic method, which allowed to estimate the fibrinogen level. It was found that βHindⅢ polymorphisms of patients with CHD were significantly associated with plasma fibrinogen levels ( r=0 7, P <0 001) and the genotypes (CT, CC) which lost cutting site for βHindⅢ enzyme showed higher plasma fibrinogen levels ( P <0 01). The results suggested that the plasma fibrinogen βHindⅢ polymorphisms may be one of the major factors of thrombosis in patients with CHD.