组合抗原金渗滤快速诊断方法在包虫病流行病学调查中的应用与评价

The assessment and application of Dot Immunogold Filtration Assay using multiple antigens in hydatid survey

目的:评价组合抗原金渗滤快速诊断方法在包虫病流行病学调查中应用的价值和意义。方法 :采用新疆包虫病临床研究所研制的“组合抗原包虫病快速诊断试剂盒 (定性 )”,对 1998～ 2 0 0 2年包虫病普查或抽查血标本进行检测 ,计算单抗原阳性率及抗原组合阳性率。结果:5次抽查样本的总阳性率均很高 (2 6.8%～ 43 .5 % ) ,而2次普查样本的总阳性率分别为 11.3 %和 8.5 %。结论:普查结果的总阳性率符合血清流行病学结果 ,Eg B特异性佳 ,但在普查中不宜单一使用 ,Em 2阳性率很低 ,与这些地区泡球蚴病的发生率远低于细粒棘球蚴病有关。

Objective: To study the value and importance of Dot Immunogold Filtration Assay (DIGFA) using multiple antigens hydatid survey. Methods: Detected blood sample from hydatid general investigation and spot test in 1998~2002 with DIGFA, and then figured out the positive rates. Results: The total positive rates in 5 spot tests were very high from 26.8% to 43.5%, but 2 general investigations were 11.3% and 8.5%. Conclusion: The total positive rates in general investigations were accordant with literature search. EgB had better speciality but not applicable along in survey. Low positive rate with Em2 was concerned with low incidences for alveolar echinoccosis in these regions. The multiple antigens DIGFA is an suitable method especially for ground hospital and field survey for hydatid disease.