新疆不同民族喉乳头状瘤与喉癌组织中不同类型HPV的检测

Detection of HPV Infection in Laryngeal Papilloma and Laryngeal Carcinoma of the Patients of Various Ethnic Groups in Xinjiang

目的 :探讨新疆部分民汉族喉乳头状瘤 (L P)和喉癌 (L C)与人乳头状瘤病毒 (HPV)感染的关系。方法 :应用聚合酶链反应 (PCR)技术 ,对 83例喉癌患者 (维吾尔族 2 7例 ,哈萨克族 4例 ,汉族 5 2例 ) ,63例喉乳头状瘤患者 (维吾尔族 3 3例 ,汉族 3 0例 ) ,2 0例声带炎性息肉 (L IP)患者 (维、汉各 10名 ) ,总共 166例分别进行 HPV 6/ 11型和 HPV 16/ 18型的检测。 结果 :(1) L P组织中 HPV 6/ 11感染的阳性检出率为 3 3 .3 % (2 1/ 63 ) ,明显高于 L C的9.6% (8/ 83 )和 L IP的 0 .0 % (0 / 2 0 ) (P <0 .0 5 ) ;(2 ) L C组织中 HPV16/ 18感染的阳性率为 3 7.3 % (3 1/ 83 )明显高于 L P的 6.3 % (4/ 63 )和 L IP的 0 .0 % (0 / 2 0 ) (P <0 .0 5 ) ;(3 )民汉之间的差异无统计学意义 (P >0 .0 5 )。 结论 :(1)民汉 L P的发生主要与 HPV6/ 11感染有关 ;L C主要与 HPV16/ 18感染关系密切。HPV感染可引起细胞基因的异常调控 。

Objective: To evaluate the role of human papillomavirus (HPV) infection in laryngeal papilloma (LP) and laryngeal carcinoma (LC) of the patients of various ethnic groups in Xinjiang. Methods: HPV6/11?HPV16/18 were detected in 166 patients of biopsy and laryngectomy specimens with LP and LC of various ethnic groups in Xinjiang, including 63 cases of LP, 83 cases of LC and 20 cases of laryngeal inflammatory polyps (LIP) by polymerase chain reaction (PCR). Results: The positive frequency of HPV 6/11 infection was higher in LP(33.3%) than in LC (9.6%) and LIP(negative) (P<0.05); the HPV 16/18 infection was 37.3% in LC and showed higher than in LP (6.3%) and LIP (negative) (P<0.05),but no statistical significance was determined in the patients of various ethnic groups in Xinjiang (P>0.05). Conclusion: HPV infection may be an important tumorigenic factor and induce some cytogenetic abnormal regulations, HPV6/11 mainly involve benign laryngeal papillomatous lesions and HPV16/18 may implicate laryngeal carcinogenesis in laryngeal epithelial tissues to develop neoplastic lesions.