天然产物3′-甲氧基葛根素溶液结构的二维NMR研究

2D NMR Study on Solution Structure of Natural Product 3′-Methoxy Puerarin

3′ 甲氧基葛根素是从常用中药野生葛根中分离提取出来的一种具有很强生物活性的异黄酮类化合物.该化合物含季碳较多,有些13CNMR的谱峰不易归属.本文利用包括氢检测异核多量子相干谱(HMQC)和氢检测远程异核多量子相干谱(HMBC)等多种二维核磁共振技术对该天然产物的13C和1HNMR谱峰进行了全归属.这些工作有助于利用二维核磁共振技术研究和确定含季碳较多的异黄酮类新化合物的结构和利用扩散相关的核磁共振新技术研究葛根的生理活性成份与靶分子相互作用.

An active isoflavone compound 3′methoxy puerarin was isolated from puerarin lobate (willd). Several twodimensional NMR new techniques, including 1Hdetected heteronuclear multiplequantum coherence and 1Hdetected multiplebond heteronuclear multiplequantum coherence, were employed to completely assign all the protons and carbons of the compound. This work solves the assignments of uncertain C3 and C1′ NMR signals in the previous literature and will be helpful to study the structures of this kind of new isoflavone compounds by 2D NMR spectroscopy and bioactive compounds in puerarin lobate directly by diffusionchemical shift 2D NMR.