绿粘帚霉诱变生物型(GVD)对三种病原真菌的拮抗作用

The Antagonism of Gliocladium virens ( GVD ) against Three Strains of Pathogenetic Fungi

本文通过对峙、对扣培养试验 ,扫描电镜观察 ,胶霉毒素粗提液的拮抗活性和热稳定性试验系统研究了绿粘帚霉诱变生物型 (GVD)对三种苗木猝倒病菌的抗生机制。结果表明 :绿粘帚霉GVD对三株病原真菌均有竞争作用 ;绿粘帚霉GVD对丝核菌、镰刀菌和腐霉有重寄生现象 ,可缠绕、侵入寄主菌丝 ,使菌丝溶解或断裂 ,变形。绿粘帚霉GVD产生的非挥发性代谢产物对病原菌的抑制率为 10 0 % ,挥发性代谢产物对丝核菌、镰刀菌和腐霉的抑制率分别为 2 6 .7%、5 1.5 %和 4 3.5 % ,胶霉毒素粗提液在 10

The antagonism of Gliocladium virens GVD against three kinds of pathogenetic fungi causing seedling damping off were studied with dual test, enclosed chamber test,scanning microscope,inhibitory activity and thermostability of crude extract of gliotoxin. The results indicated that GVD was competitive and parasitic to Rhizoctonia solani, Fusarium spp and Pythium spp ,GVD coiled itself round hyphae of the above fungi, made their hyphae broken off,and penetrated their hyphae .The inhibitory rates of nonvolatilizable metabolites from GVD were 100%;the inhibitory rates of volatilizable metabolites from GVD were 26.7%?51.5% and 43.5% against Rhizoctonia?Fusariu?Pythium . The thermostability of crude extract of gliotoxin was under 100℃. The antagonism of Gliocladium virens GVD against Rhizoctonia solani,Fusarium spp and Pythium spp is evident.