电针对血管性痴呆模型大鼠学习记忆行为及脑血红素氧化酶活性的影响

Effects of Electroacupuncture on Learning and Cerebral Heme Oxygenase Activity in Rats with Vascular Dementia

【目的】探讨电针对血管性痴呆(VD)模型大鼠学习记忆行为及血红素氧化酶(HO)的影响。【方法】60只大鼠随机分为假手术组、模型组、电针组和尼莫通组。用四血管阻断方法制作拟VD大鼠,造模后第10d电针组电针百会、大椎穴,1次/d;尼莫通组给予尼莫通12mg/kg稀释后,按20mL/kg灌胃,1次/d,共15d。治疗后均以水迷宫检测大鼠学习记忆行为情况,并检测皮质和海马HO活性。【结果】电针组模型大鼠逃避潜伏期明显短于模型组(P<0.01),电针组与尼莫通组之间无显著性差异(P>0.05);电针组在原平台象限跨越平台次数显著多于其余3个象限,而且多于模型组在原平台象限跨越平台次数。在皮质及海马,电针组和尼莫通组HO活性显著低于模型组(P<0.01)。【结论】电针能改善VD大鼠学习记忆能力.调节皮质及海马的HO活性。

[Objective] To observe the effect of electroacupuncture (EA) on learning and cerebral heme oxygenase (HO) activity in rats with vascular dementia (VD). [Methods] Sixty rats were randomly allocated to mimic group (Group A), EA group (Group B), nimotone group (Group C) and model group (Group D) . Rat models of VD were established by 4 - vessel occulusion (4 - VO) method. Ten days after modeling, Group B was trested with EA on Baihui (GV20) and Dazhui (GV14), one time per day; Group C with nimotone, 20ml/kg body weight, ig, qd. The treatment course lasted 15 days. And then the learning was estimated with Morris water maze and HO activity in cortex and hippocampus (HC) of rats was also measured. [Results] Mean escape latency within 6 days in EA group was shorter than that in model group ( P<0.01) but did not differ from that in nimotone group (P > 0.05). In EA group, the swimming times in the original platform quadrant were more than that in the right, left or opposite quadrant and also more than that in the original platform quadrant in the model group (P < 0.01). HO activity in EA group and nimotone group were lower than that in model group (P<0.01). [Conclusion] EA can improve the learning and regulate HO activity in cortex and hippocampus of rats with vascular dementia.