摘要
介绍运用液氮冻存的大鼠心室肌来分离心室肌细胞的具体方法,并初步分析了该方法的机理、科学性、实用性。认为该方法具有无需特殊昂贵设备、简单易行、消耗低廉、耗时短、适于大样本研究的特点,可望成为一种基础的实验方法。
Separation of myocardiocytes from adult rat ventricular myocardium frozen by ultralow - temperature liquid nitrogen was introduced, and its mechanism was discussed preliminarily as well. The results indicate that this is a scientific and practical method, which is simple, inexpensive, taking less time and in no need of special apparatus. It is expected to be a basic technique for large-sample cardiological research.
出处
《广州中医药大学学报》
CAS
2002年第4期289-291,共3页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
广东省中医药管理局资助课题(编号:B99049)
关键词
成年
大鼠
心室肌细胞
超低温液氮保存
分离法
VENTRICULAR MYOCARDIOCYTES
CRYOPRESERVATION
CELL SEPARATION
FLOW CYTOMETRY