保心康对心肌肥大型心力衰竭大鼠血管平滑肌环核苷酸水平的影响

Effect of Bao Xin Kang on Cyclic Nucleoside Phosphate Level in Vascular Smooth Muscle of Rats with Myocardial Hypertrophic Heart Failure

【目的】探讨保心康(由人参、附子、毛冬青等组成)对心肌肥大型心力衰竭大鼠血管平滑肌环磷酸腺苷(cAMP)和环磷酸鸟苷(cGMP)水平的影响及其作用机理。【方法】大鼠皮下注射异丙肾上腺素共13d以制备心肌肥大型心力衰竭模型,采用放射免疫分析法观察各组动物血管平滑肌。cAMP和cGMP水平的变化,同时对大鼠体重和心、肝、肺分别称重,并对心、肝、肺脏器进行组织形态学现察。【结果】模型组大鼠血管平滑肌cAMP、cGMP含量及心脏和肝脏的指数均比正常对照组显著升高(P<0.01)。而中药保心康能明显降低cAMP,和cGMP含量及心脏和肝脏的指数(P<0.05或P<0.01)。组织形态学观察显示保心康能改善病理状态下大鼠心、肝脏形态结构。【结论】保心康对实验性心肌肥大型心力衰竭有较好的防治作用,其作用机理与cAMP、cGMP的调节有关。

[Objective] To investigate the effect of Bao Xin Kang (BXK) on levels of cyclic adenosine monophosphate (cGMP) and cyclic guanosine monophosphate (cAMP) in vascular smooth muscle of rats with myocatdial hypertrophic heart failure and to explore its mechanism. [Methods] Fifty rats were randomly allocated to five groups: normal control group (Group A) , model group (Group B) , digoxin group (Group C), large - dose BXK group (Group D) and small - dose BXK group (Group E). Rat models with myocardial hypertrophic heart failure were established by subcutaneous injection of isoprenaline for 13 days. Radioimmunoassay was used to measure the levels of cAMP, cGMP and the ratio of cAMP/cGMP in vascular smooth muscle. The body weight and the weight of heart, liver and lung were measured and his-tomorphologic features of heart, liver and lung also examined. [Results] The levels of cAMP and cGMP and the heart index (heart weight /body weight) and the liver index (liver weight/body weight) in Group B were increased as compared with those in Group A (P < 0.01); the levels of cAMP and cGMP in Group E and the heart index and the liver index in Group D and Group E were decreased as compared with those in Group B(P<0.05orP<0.01). And BXK could also improve the histomorphologic features of heart and liver. [Conclusion] BXK exerts a better effect in preventing and treating experimental myocardial hypertrophic heart failure and its mechanism may be related to the regulation of the levels of cAMP and cGMP.