吲达帕胺/硫氮酮对缺血-再灌注心肌能量代谢的影响

Effect of indapamide and diltiazem on energy metabolism of the myocardium after ischemia-reperfusion

目的 探讨吲达帕胺 (Indapamide)、硫氮艹卓 酮 (Diltiazem)保护缺血 -再灌注心肌的作用机制。方法 SD大鼠 4 5只 ,随机分为对照组、吲达帕胺组、硫氮 艹卓 酮组。比较 3组动物在再灌注前、后能量代谢的变化。结果 对照组在心肌再灌注 30、6 0min后与缺血前比较 ,三磷酸腺苷 (ATP)含量明显降低 (P <0 0 5 )。吲达帕胺组、硫氮艹卓 酮组在再灌注 30、6 0min后与对照组比较 ,心肌ATP含量明显升高(P <0 0 5 )。结论 吲达帕胺、硫氮艹卓 酮的心肌保护作用与其增加心肌细胞能量贮备等有关。

Objective: To study the protective effect of indapamide and diltiazem on reperfusion injury. Methods: 45 healthy Sprague-Dawley rats were divided into 3 groups at random: indapamide group, diltiazem group and control group. Isolated rat hearts were perfused by Langendorff apparatus. Assessments of ATP were done 30 minutes after ischemia and 30 minutes or 60 minutes after reperfusion. Results: (1) 30 minutes after ischemia arrest, and 30 minutes or 60 minutes after reperfusion, ATP in all groups was found significantly lower than the prearrest levels. (2) The indapamide group and diltiazem group had higher ATP levels at 30 minutes after ischemia, and significantly, higher ATP levels at 30 minutes or 60 minutes after reperfusion. Conclusion: Indapamide and diltiazem both have protective effect on reperfused myocardium. Its mechanism may be through increasing energy store.