康莱特诱导肿瘤细胞凋亡的实验研究

Mechanisms of Kanglaite Induced Apoptosis in Human Cancer Cells

目的:探讨康莱特的抗肿瘤机制,特别是凋亡的作用机制。方法:应用形态学方法、流式细胞术、DNA凝胶电泳、AnnexinV标记等方法检测细胞凋亡的发生。应用RT-PCR检测凋亡相关基因Fas、bc1-2、c-myc表达的变化。结果:康莱特对人肺腺癌细胞A549和人早幼粒细胞性白血病细胞HL60的生长均有抑制作用,引起细胞死亡。康莱特对A549细胞诱导凋亡的反应并不明显,而对HL60细胞有明显的凋亡。在HL60凋亡过程中,凋亡相关基因Fas转录水平比用药前增强,c-myc和bc1-2含量无变化。结论:凋亡与坏死同为康莱特抑瘤的机制,但不同细胞系凋亡所发生的比例不同。提示不同肿瘤,其凋亡规律亦不同,为康莱特的临床应用提供了理论依据。

To study the anti-tumor effect of Kanglaite(KLT)and the mechanisms of its induction of apoptosis of cancer cells.Morphological study,DNA gel electrophoresis,flow cytome try and fluorescein labelled Annexin V were used to detect the apoptosis and RT-PCR was used to de-tect the apoptosis related changes of gene Fas,bcl-2and c-myc.Results:The result showed that KLT could inhibit the proliferation of both HL-60and A549cells;KLT could also induce apoptosis of HL-60cells in which Fas gene took place in the process,but KLT did not act significantly on A549cells.Conclusion:KLT induced apoptosis and necrosis of human cnacer cells is the key role for its killing action on cancer cells in the treatment of cancer.