慢性乙型肝炎患者外周血树突状细胞免疫功能的研究

Study on the immune function of peripheral blood dendritic cells from chronic B patients

目的 研究慢性乙型肝炎患者外周血树突状细胞 (DC)表面分子HLA -DR、CD1a、CD80 、CD86表达水平及其与DC免疫功能的相关性。方法 临床确诊的慢性乙型肝炎轻度患者 1 0例 ,从其外周血中分离单个核细胞 ,体外培养扩增DC ,流式细胞仪检测DC表型HLA -DR、CD1a、CD80 、CD86。以健康成人 1 0例作为对照。结果 慢性乙型肝炎患者DC表面HLA -DR、CD1a、CD80 、CD86分别为 :48.63± 6.34,2 7.73±7.1 9,2 1 .0 5± 8.33,1 8.5 8± 5 .5 1 ,明显低于对照组 :83.1 1± 6.46,5 2 .80± 1 1 .70 ,36.1 1± 7.5 5 ,40 .83± 5 .48,两者相比差异显著 (P <0 .0 0 1 )。结论 慢性乙型肝炎患者外周血DC表面HLA -DR、CD1a、CD80 、CD86表达水平下降 。

Objective To study the property of dendritic cells(DCs) of peripheral blood monocytes (PBMC) in patients with chronic HBV infection and identify the relations with immune function.Methods Ten patients with persistent HBV infection were included in this study, ten healthy subjects being used as a control group. The peripheral blood mononuclear cells(PBMC) were incubated and induced into mature dendritic cells in the RPMI-1640 medium in the presence of cytokines GM-CSF,IL-4 and TNF-a for a total 12 days. Then using FCM to study the expression levels of HLA-DR,CD 1a ,CD 80 and CD 86 .Results The expression levels of HLA-DR,CD 1a ,CD 80 and CD 86 on DC surface from patients were significantly lower than that from healthy individuals (48.63±6.34 vs 83.11±6.46 for HLA-DR,27.73±7.19 vs 52.80±11.70 for CD 1a ,21.05±8.33 vs 36.11±7.55 for CD 80 ,18.58±5.51 vs 40.83±5.48 for CD 86 ), P <0.001.Conclusion The patients with chronic HBV infection have the defective function and immature phenotype of dendritic cells ,which may be associated with the inability of efficient presentation of HBV antigen to host immune system for the clearance of HBV.