测定系统性红斑狼疮患者ANCA的临床意义

Clinical significance of ANCA in the SLE patients

目的 探讨抗中性粒细胞胞浆抗体 (ANCA)在系统性红斑狼疮 (SLE)中的临床意义。方法 通过间接免疫荧光法 (IIF法 )及免疫印迹方法 ,检测 61例SLE患者血清中ANCA、ANA、抗ds DNA及抗u1 RNP等自身抗体。结果 用IIF法检测时 ,ANCA在SLE中的阳性率是 40 .9% ,均为核周型ANCA(P AN CA) ,ANA在SLE中的阳性率是 91 .8%。 2 5例ANCA阳性患者的ANA均为阳性。在ANCA阳性组抗ds DNA抗体的阳性率明显高于ANCA阴性组 ( χ2 =5 .6,P <0 .0 5 )。结论 ANA能干扰ANCA的结果判断 ,若采用单一粒细胞的底物片来判断SLE病人是否合并ANCA是不合适的。

Objective To study clinical significance of the detection of ANCA in SLE.Methods By indirect immunofluorescence(IIF)and western blotting autoantibody such as ANCA?ANA?anti-ds-DNA antibody and anti u 1 RNP antibody was detected in the serum of 61 patients.Results To detect by IIF,the positive rate of ANCA in SLE is 40.9% and the type of ANCA are all peripheral-ANCA(P-ANCA);the positive rate of ANA is 91.8%. ANA were also positive in 25 patients whose ANCA were positive. In the Group which ANCA is positive,the positive rate of anti ds DNA autoantibody is higher than the negetive one( χ 2=5.6, P <0.05).Conclusions ANA can confuse the detection result of ANCA . It won't be suitable that jugding whether SLE commingled ANCA by single substrate piece of granulocyte. Detecting ANCA in the SLE patient may help to judge the avidity of this disease