新疆细粒棘球绦虫DNA限制性酶切片段长度多态性的初步分析

DNA RFLP Analysis of Echinococcus granulosus from Xinjiang: A Preliminary Study

应用光敏生物素标记细粒棘球绦虫特异性DNA探针pEG18,对新疆5个不同地区绵羊、1例肝包虫病人、阿勒泰地区牛细粒棘球绦虫原头节以及羊源、牛源细粒棘球绦虫成虫DNA进行限制性酶切谱和Southern杂交分析。牛源、羊源样本经EcoRI酶切后产生的杂交图型基本上是一致的。BamHI酶切后产生的杂交结果,在新疆5个不同地区绵羊感染的细粒棘球绦虫没有发现差别,而人源和牛源细粒棘球绦虫DNA产生的杂交图型与绵羊源细粒棘球绦虫DNA产生的杂交图型大体上是一致的,但又存在着各自的差异。与绵羊样本相比,人源细粒棘球绦虫产生的杂交图型约在8.3kb处明显可见一条杂交带,而牛源细粒棘球绦虫约在3.5kb处有一条清晰的杂交带。这些差异的意义有待进一步研究。用光敏生物素代替同位素标记DNA探针,简便易行,稳定可靠,可取得满意的结果,在边远的地区是一种值得推广的方法。

The DNA RFLP of the protoscolex and adult worm of Echinococcus granulosus of sheep and cattle origin from different areas of Xinjiang were analysed using a pecifice DNA probe PEG 18 (Kindly sent by Dr.D.P.MacManus) labelled by phetobiotin. The RFLP patterns with the samples of sheep and cattle origin digested by restriction enzyme EcoRI were similar. The hybridisation patterns of DNA samples of sheep origin from five different areas were also identical after Bam HI digestion. While the RFLP patterns with the DNA of human (a liver hydatidosis patient of the Siboo nationality from Chabuchar Siboo Autonomous County) and cattle origin were different as compared with the DNA samples from sheep, there were a particular hybridization bands at the position of 8.3 kb and 3.5 kb, respectively. The significance of these differences remains to be studied, The labelling of DNA probe with photobiotin is a convenent and stable method and is valuable particularly in the remote areas.