环氧乙烷消毒的明胶海绵对耳螺旋神经节细胞的毒性研究

Ototoxic study spiral ganglion neurons in the pig guinea with ethylene oxide remained in absorbable gelatin sponge

目的 :研究并观察环氧乙烷消毒的明胶海绵对耳螺旋神经节细胞的毒性作用。方法 :用比色法测定明胶海绵的环氧乙烷残留量 ,通过透射电镜观察明胶海绵放置豚鼠听泡内 3个月后 ,耳蜗螺旋神经节细胞的超微结构变化。结果 :明胶海绵的环氧乙烷残留量平均为 4 38.32mg。螺旋神经节超微结构显示 :I型螺旋神经节细胞线粒体基质电子密度增大 ,嵴模糊不清 ,细胞质内出现空泡样结构。部分神经节细胞及其外周的卫星细胞溶解破坏 ,呈典型细胞死亡征象。卫星细胞髓鞘松解 ,出现空化。结论 :明胶海绵的环氧乙烷残留量高出国家标准约4 0倍 。

Objective:To measure ethylene oxide remained in absorbable gelatin sponge, and to observe change of ultrastructure in spiral ganglion neurons after deposited in otic vesicle of pig guinea with ethylene oxide sterilized absorbable gelatin sponge.Method:To use comparative colour technique measureing ethylene oxide remained in absorbable gelatin sponge, by means of transmission electron microscope to observe change of ultrastructure in spiral ganglion neurons after 3 months deposited in otic vesicle of pig guinea with ethylene oxide sterilized zbsorbable gelatin sponge.Result:The mean value of ethylene oxide remained in absorbable gelatin sponge were 438.32 mg, while nation standard are less 10 mg, FDA standard(1978) less 25 mg. Ultrastructural appearance showed condensed matric electron dense of spiral ganglion mitochondria and dark crista of mitochondria, vacuolated cytoplasm. Utrastructural observation also demonstrated dissolution or necrosis of ganglion cells,satellite cells, and vacuolated myelin. These changes were near Donson reported(1997) intracochlear perfusion with aminoglycosides.Conclusion: The mean value of ethylene oxide remained in absorbable gelatin sponge were 40 times nation standard. Toxic changes of ethylene oxide remained in absorbable gelatin sponge in spiral ganglion neurons were similar to intracochlear perfusion with aminoglycosides.