经腹腔诱导免疫耐受抑制EAE的实验研究

Suppression of Experimental Autoimmune Encephalomyelitis by Peritoneoinduced Immunologic Tolerance

目的 通过建立实验性自身免疫性脑脊髓炎 (EAE)动物模型 ,研究经腹腔诱导免疫耐受的方法及机制。 方法 腹腔内注射可溶性髓鞘碱性蛋白 (MBP)或转输可溶性 MBP致敏的腹腔抗原提呈细胞 (MBP-APC) ,观察各组动物 EAE的发病情况 ,检测各组动物迟发型超敏反应 (DTH)和淋巴细胞体外增殖应答。结果 腹腔内注射 MBP及 MBP-APC的动物发病率明显低于 EAE组 ,临床症状减轻 ,由 MBP引起的 DTH和体外特异的MBP淋巴细胞增殖反应也明显降低。结论 腹腔注射 MBP或 MBP-APC可引起黏膜耐受 ,抑制 EAE的发生。目的 通过建立实验性自身免疫性脑脊髓炎 (EAE)动物模型 ,研究经腹腔诱导免疫耐受的方法及机制。 方法 腹腔内注射可溶性髓鞘碱性蛋白 (MBP)或转输可溶性 MBP致敏的腹腔抗原提呈细胞 (MBP-APC) ,观察各组动物 EAE的发病情况 ,检测各组动物迟发型超敏反应 (DTH)和淋巴细胞体外增殖应答。结果 腹腔内注射 MBP及 MBP-APC的动物发病率明显低于 EAE组 ,临床症状减轻 ,由 MBP引起的 DTH和体外特异的MBP淋巴细胞增殖反应也明显降低。结论 腹腔注射 MBP或 MBP-APC可引起黏膜耐受 ,抑制 EAE的发生。

Objective To investigate the suppressive effect of immunologic tolerance induced peritoneoly on experimental autoimmune encephalomyelitis (EAE). Methods The animals were administrated with the soluble myelin basic protein (MBP) or transferred MBP sensitizing antigen presenting cells (MBP APC) intraperitoneally (ip). The clinical symptoms of EAE were observed. The delayed type hypersensitivity(DTH) and proliferative responses to MBP in vitro were tested. Results The incidence of EAE and the clinical symptoms of encephalomyelitis were obviously lower both in the MBP(ip) and MBP APC(ip) group than that of EAE group, otherwise,DTH responses and the antigen specific proliferative responses to MBP in vitro were markedly suppressed. Conclusions EAE can be suppressed by immunologic tolerance induced with intraperitoneal administration of MBP or MBP APC.