摘要
用明胶酶谱的方法检查了野生型和Smad3ex8 ex8纯合突变小鼠血清中基质金属蛋白酶(MMP9)的活性 .发现突变小鼠血清中MMP9的含量较正常小鼠的明显增高 ,提示SMAD3有抑制MMP9表达的功能 .通过细胞转染实验证实 ,TGF β1和野生型的SMAD3可以抑制COS7细胞分泌MMP9,而C端缺失的突变型Smad3基因过表达可以解除这种抑制作用 ,说明SMAD3介导TGF β1信号抑制MMP9在COS7细胞中的表达 .
The amount of MMP9 in serum of wild type and Smad 3 ex8/ex8 homozygous mice was examined by gelatin zymography. The results showed that the amount of MMP9 in serum of mutant mice is higher than that of wild type mice. It suggested that SMAD3 could inhibit the expression of MMP9, which was verified by transient cell transfection experiments. The expression of MMP9 in COS7 cells decreased about 20% ( n=3, P <0.05) when treated with TGF β1 and almost stopped when transfected with wild type Smad 3 gene, suggesting that the expression of MMP9 could be inhibited by TGF β1 and the over expression of wild type Smad 3 gene. The expression of MMP9 in the samples transfected with C terminal truncated Smad 3 gene was similar to the control (n=3, P <0.05),indicating that the over expression of the C terminal truncated Smad 3 gene could block this inhibition. The data suggested that SMAD3 could mediate TGF β1 to inhibit the expression of MMP9 in COS7 cells.
出处
《中国生物化学与分子生物学报》
CAS
CSCD
北大核心
2002年第6期684-687,共4页
Chinese Journal of Biochemistry and Molecular Biology
基金
国家自然科学基金项目 (No.3 9970 413 )
国家杰出青年科学基金(No .3 0 0 2 5 0 2 8)资助
