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梅花鹿卵泡刺激素β亚基cDNA的分子克隆与序列分析 被引量:1

Cloning and Sequencing of cDNA for β-Subunit of Sika Follicle Stimulating Hormone
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摘要 Total RNA was prepared from pituitary gland of new butchered sika cDNA was synthesized by RT\|PCR and used as the template in PCR amplification for β\|subunit gene of sika follicle stimulating hormone(FSH). The PCR product was 390 bp in 1\^2% agarose gel electrophoresis which just was of the same length as the predicted FSH β\|subunit gene. Cloned to pMD\|18\|T\|Vector, selected 2 positive recombinants at random and analyzed its sequence. The comparison of this amino acid sequence of FSH β\|subunit with some other mammalian showed that it has higher homology with horse, pig, dog and lower homology with rat. Although FSH β\|subunit gene shows species specificity, it is quite conserved among mammalians. Total RNA was prepared from pituitary gland of new butchered sika cDNA was synthesized by RT\|PCR and used as the template in PCR amplification for β\|subunit gene of sika follicle stimulating hormone(FSH). The PCR product was 390 bp in 1\^2% agarose gel electrophoresis which just was of the same length as the predicted FSH β\|subunit gene. Cloned to pMD\|18\|T\|Vector, selected 2 positive recombinants at random and analyzed its sequence. The comparison of this amino acid sequence of FSH β\|subunit with some other mammalian showed that it has higher homology with horse, pig, dog and lower homology with rat. Although FSH β\|subunit gene shows species specificity, it is quite conserved among mammalians.
出处 《中国生物化学与分子生物学报》 CAS CSCD 北大核心 2002年第6期783-785,共3页 Chinese Journal of Biochemistry and Molecular Biology
关键词 梅花鹿 卵泡刺激素β亚基 CDNA 分子克隆 序列分析 sika follicle stimulating hormone, β\|subunit, cDNA, sequence analysis
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  • 1Wang H,Mol Endocrinol,1992年,6卷,3期,320页
  • 2刘殿芹,国外医学.计划生育分册,1996年,15卷,2期,124页
  • 3周清,国外医学.计划生育分册,1996年,15卷,3期,174页
  • 4隋龙,国外医学.妇产科学分册,1995年,22卷,5期,259页
  • 5邢爱棣,国外医学.妇产科学分册,1995年,22卷,3期,179页
  • 6焦丽娅,国外医学.计划生育分册,1994年,13卷,1期,56页
  • 7尹德钟,贾锋,张永清,高文玉,周顺伍,齐顺章.由大肠杆菌中提取重组猪生长激素的研究[J].生物化学杂志,1992,8(3):353-357. 被引量:3

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