摘要
为提高造模成功率提供参考,对大鼠大脑中动脉(MCA)距颈内动脉(ICA)起始部的距离进行了测定,并观察插线深度与MCA起始部距离及造模成功率的关系。栓线法制作大鼠大脑中动脉缺血(MCAO)模型,插线深度组,进行动脉灌注显色铸型,测量MCA起始部距ICA起始部的距离;脑梗塞体积组,计算机图像分析脑梗塞区面积,计算脑梗塞体积。结果发现,MCA起始部到ICA起始部的距离在1.55～1.65cm之间,栓线插入深度小于等于1.60cm的造模成功率为28.57％,插入深度大于1.60 cm的造模成功率为83.33％。二者比较有显著差异(P<0.05)。提示栓线法制作大鼠MCAO模型的插线深度起码应达到1.65 cm。
To determine the distance between the middle cerebral artery (MCA) and the origin of internal artery (ICA) in rats and observe the relationships of the inserted thread length to the MCA origin and to the successful model-making rate. :A. thread occlusion method was used to make a MCAO model. Artery perfusion color casting was performed, and the distance between the origin of MCA and the origin of ICA was measured in the inserted thread length group. The cerebral infarct area was analysised and the cerebral infarct volume was calculated by computer image processing in the infarct volume group. The result showed that the distance between MCA and ICA ranged from 1.55 cm to 1. 65 cm. On the condition that the .length of the inserted nylon thread was less than or equal to 1. 60 cm, the successful model-making rate was 28. 57%; if the length was more than 1. 60 cm, the successful rate was 83. 33%(P<0. 05). Conclusion ?If we use a thread occlusion method to make a rat MCAO model, the length of inserted nylon thread must be no less than 1. 65 cm.
出处
《上海实验动物科学》
2002年第4期209-212,共4页
Shanghai Laboratory Animal Science
基金
国家自然科学基金(No.39730510)
