大鼠急性呼吸窘迫综合征动物模型的建立

Establishment of Acute Respiratory Distress Syndrom Animal Model in Rats

目的 建立大鼠急性呼吸窘迫综合征动物模型 (ARDS)。方法 选取KM小鼠SD大鼠 (雌雄不限 ) ,分为正常对照组及百草枯组 ,小鼠按每公斤体重 10 0mg ,大鼠按每公斤体重 12 0mg一次灌胃给药。再分 2 4h、4 8h、72h组 ,小鼠每组 10只股动脉采血用于血气分析 ,取肺测定肺系数 ,全肺拍照进行对照 ,大鼠每组 10只取肺石腊包埋 ,病理切片。结果 ①整体外观变化 随着给药时间延长 ,呼吸变得越来越困难、消瘦、咳嗽 ,2 4h后鼻腔有淡红色分泌物 ,食欲渐进性下降。②肺外观观察炎症渐进性发展 ,从正常粉红色发展到最后呈肝样变。③血气变化 随染毒时间延长 ,血液pH值逐渐降低 ,PaCO2 逐渐升高 ,PaO2 逐渐下降 ,肺系数逐渐增大 ,组间数据进行t检验p <0 0 1,差异有显著性。④病理切片观察肺部呈典型炎症病理变化过程 ,最终肺泡内形成典型透明膜。结论 百草枯染毒成功制造鼠ARDS模型。

ObjectiveTo establish the ARDS animal model in rats. MethodKM mice and SD rats were divided into control group and paraquat -exposed groups (with a single dose of 100mg/kg in mouse and 120mg/kg in rat). Each group was subdivided into three smaller groups (24 -hour group, 48 -hour group, and 72 -hour group). Blood was collected from the femoral artery of mouse for blood gases analysis. Tissues from lung of rats were embedded in paraffin for histological analysis. Result1.After the paraquat was given, respiration movements of the animals were getting more and more difficult with cough, pink excretions in nasal cavity, progressively decreased appetite, and weight loss. 2.Lung changed from pink color to hepatization in gross view. 3.By blood gases analysis, the blood PH value and PaO 2 gradually decreased, while PaCO 2 and pulmonary coefficient went in the other way. Statistical analysis (t -test) showed an obvious difference between experimental groups and control group ( P <0 .01). 4.Histological section showed a typical inflammation. Transparent membrane formed in the pulmonary alveoli. ConclusionARDS animal model can be successfully produced by means of exposure to paraquat.