离体马尾松胚萌发过程中IAA及其氧化酶和生物大分子合成的变化

Changes of the Biosynthesis of IAA and Its Oxidase and Macromolecules in Pinus massoniana Embryos Cultured in Vitro during Germination

离体马尾松 (PinusmassonianaLamb .)胚萌发过程中 ,过氧化物酶活性逐步增强 ,10h和 4 0h出现两个剧增期。IAA氧化酶活性逐渐降低 ,10h和 4 0h出现两个剧降期。IAA含量则一直维持上升趋势。同位素前体标记检测表明 ,蛋白质合成速率在 5h明显提高 ,15h速率加快 ,30～ 4 0h出现一个平缓阶段 ,4 0h后剧增 ;RNA合成速率 ,在 15h以前处于低水平 ,15h开始加快 ,2 5～ 30h出现一个平缓阶段 ,30h后速度提高 ;DNA合成速率在 4 0h才明显加快。

During germination of Pinus massoniana embryos cultured in vitro, the activity of peroxidase was increased drastically in 10?h and 40?h. The activity of IAA oxidase was decreased drastically in 10?h and 40?h during germination. The experimental results of radioactive isotopes showed: The synthesis rate of protein was evidently raised in 5?h and was quickly raised in 15?h, then the rate was drastically raised after 40?h. The synthesis rate of RNA was lower before 15?h and was quickly raised in 15?h, then the rate was drastically raised after 30?h.The synthesis rate of DNA was evidently raised after 40?h.