大连近海河流弧菌Ⅱ噬菌体的分离与研究

ISOLATION AND CHARACTERIZATION OF BACTERIOPHAGES OF VIBRIO FLUVIALS Ⅱ IN THE COATAL WATERS OF YELLOW SEA ,DALIAN

1996年、 1 997年两个夏季 (5～ 8月 )、从大连市水产养殖公司所属的不同的皱纹盘鲍养殖区采集海水及海底淤积物样品共 76个。以河流弧菌Ⅱ (Vibriofluvialis)为指示菌 ,从这些样品中分离到 1 9株噬菌体。对它们进行了电子显微镜的观察 ,结果发现它们均属于BradleyA型。进一步的血清型分析表明 ,这 1 9株噬菌体分别属于 4个血清型 ,并对这 4个血清型的代表株VP1 ,VP2 ,VP4及VP8进行了寄主范围、一次生长曲线测定及其他生物学特性的初步分析。

Using Vibrio fluvialis Ⅱ as host bacteria, 19 bacteriophages have been isolated from the 76 samples which were collected from Haliotis discus hannai ～growing seawater in Dalian marine culture company Dalian, liaoning province from May in 1996 to August I 1997. Ultrastructure of 19 bacteriophages were observed with electron to Bradley the results showed that of these bacteriophages belonged to Bradley A type, they have hexagonal heads of bacteriophages were identified with VP1,VP2,VP4,VP8 as representatives respectively. The phages remain stable at pH6. 0～10. 0, moreover VP2,VP4 and VP8 are rather stable at basic pHs. Although the characterization of heat inactivation course of VP4 is different from others, four phages are sensitive to heat and inactivated at 80℃ in 5 minutes. One step growth experiment showed that the eclipse period of VP1,VP2,VP4,VP8 are sensitive to heat and the eclipse period of VP1, VP2, VP4, VP8 are 42, 30, 46, 28 minutes. In this experiment we have isolated at least four different types phages, it suggest that in fact there is a population of phages in the seawater environment. The result of this study provided a way to find the potential value of phages as an indicator of pathogenic microorganisms Vibrio fluvilis Ⅱ in marine environment.