白藜芦醇诱导CNE-2Z细胞凋亡过程中caspase-3的活化

Activation of caspase-3 during resveratrol (Res)-ind uced apoptosis of CNE-2Z cells

目的 探讨白藜芦醇 (Res)诱导鼻咽癌细胞CNE 2Z凋亡过程中是否有caspase 3的活化。方法 用Western blot分析caspase 3酶原的变化 ,半定量RT PCR检测caspase 3mRNA水平的改变 ,比色法测定caspase 3的相对活性。结果 用0、2 5、5 0、10 0、2 0 0 μmol LRes处理CNE 2Z细胞 2 4h ,随药物浓度的增加 ,caspase 3酶原的蛋白水平减少 ,caspase 3的mRNA水平增加 (P <0 .0 1)。用 10 0 μmol LRes分别处理细胞 1、2、4、8、12、2 4h ,caspase 3活性于 2h开始升高 ,12h达高峰 (为对照组的6 .6倍 ,P <0 .0 1) ,2 4h仍高于对照组 (P <0 .0 1) ;用不同浓度的Res处理细胞 12h ,caspase 3活性呈浓度依赖性的升高。结论 Res诱导CNE 2Z细胞凋亡过程中有caspase

ObjectiveTo study whether caspase 3 is activated duri ng resveratrol(Res) induced apoptosis of nasopharyngeal carcinoma cells CNE 2Z. MethodsThe changes of pro caspase 3 were analyzed by Wester n blot. The changes of caspase 3 mRNA was detected by semi quantitative RT PCR. Caspase 3 relative activity was determined by colorimetric assay. Results After CNE 2Z cells were treated with 0,25,50,100,2 00 μmol/L Res for 24 h respectively,the level of pro caspase 3 protein decr eased as concentration increased. The level of caspase 3 mRNA increased as conc entration inc reased. When the cells were treated with 100 μmol/L Res for 1,2,4,8,12,24 h respectively,the caspase 3 activity began to rise at 2 h,reached the peak at 12 h ( 6.6 times of that of the control group,P<0.01) and was still much higher than that of the control group at 24 h (P<0.01).After th e cells were treated with Res of different concentrations for 12 h, caspase 3 a ctivity increased in a concentration dependent manner. Conclusion Caspase 3 is activated during Res induced apoptosis of CNE 2Z cells.