滇中稻瘟病菌多样性及分布

Genetic Diversity and Distribution of Magnaporthe grisea in the Central Area of Yunnan Province

对采自云南水稻主产区 15县的 2 36个稻瘟病菌株进行了rep -PCR (repetitiveelement basedpolymerasechainre action)指纹分析 ,应用STATISTICA 5 0软件UPGRAM程序构建了 134个代表菌株的DNA指纹聚类图。依 1 75遗传相似距将供试菌株划分为 8个遗传型群 ,第 1,2 ,4群为优势群。各遗传型群具有一定的地域性 ,同一块稻田同一寄主品种上稻瘟病菌遗传型群基本一致 ,但有时包含不同的遗传型 ,甚至同一病斑存在遗传型差异。

Two hundred and thirty six isolates of Magnaporthe grisea collected from 15 rice growing counties of Yunnan Province were assessed for DNA polymorphism exploiting the repetitive element based PCR (rep-PCR). Cluster analysis generated a linkage graph of the DNA fingerprints from 134 representative isolates using the STATISTICAL 5.0 software with unweighted pair grouped average program. All the isolates were classified into 8 genetic linkage groups at the level of 1 75 linkage distance, of which the No.1, No.2 and No.4 were the dorminant groups. The results indicated that isolates in a certain area generally belonged to one genetic group and the isolates from the same plot and host cultivar mostly shared one genetic group though they sometimes showed difference, or even different genetic group for those strains from one lesion. The genetic linkage group was obviously correlated to the host rice cultivar.