一株具有抗真菌活性的食酸丛毛单胞菌的分离和鉴定

Identification of an isolate of Delftia acidovorans with anti-fungi activity

在土壤中分离到一株细菌STRA ,菌块测定法显示该菌可显著抑制玉米小斑病菌和稻恶苗病菌 ,具有抗真菌活性。该菌可以在SFM和LB等多种培养基上生长 ,且菌落生长无明显终止现象 ;革兰氏染色为阴性 ,电镜观察可见 2～ 4根鞭毛。 (G +C) %在 5 9%～ 6 7%范围内。生化指标的检测显示该菌株对所测试的多数糖类不能发酵 ,经BioMerieuxVitek的革兰氏阴性细菌检测试验卡 (GNI)鉴定为食酸丛毛单胞菌 (Delftiaacidovorans)。设计两对简并引物对其 16SRNA进行PCR扩增 ,16SRNA的序列及其相关生物信息学分析结果进一步确证生化鉴定结果。该分离株的抗真菌物质的特性鉴定。

A bacterial strain named STR A was isolated from soil,which can remarkably inhibit the growth of Cochliobolus heterostrophus and Gibberella fujikuroi.The isolate can be cultivated on SFM and LB agar,etc.The colony can growth on these medium without halt.The bacterium is gram negative and can be viewed with 2?4 flagellas under electron microscope.The(G+C)% was determined between 59% and 67%.The strain can not ferment most sugars in the biochemical test and it was identified as Delftia acidovorans with the gram negative identification card(GNI)of BioMerieux Vitek company.Two pairs of primers were designed for PCR of its 16S RNA.The sequence analysis of 16S RNA sequence with online software further confirmed this results.Purification,analysis and anti fungi mechanism of the anti fungi material will be studied in the future.