利用RAPD技术进行杂交油菜杂油59品种鉴定和纯度分析

Identification and Purity Analysis on Hybrid Rapeseed 'Hybrid 59'Study with RAPD Technique

研究建立了简便快速微量提取油菜DNA的方法 ,并从 370个随机引物中筛选出 5个可明显区分CMS杂交油菜品种杂油 5 9及其亲本系的引物。利用两个引物将杂油 5 9杂种和目前生产上应用或即将应用的 2 2个品种组合或自交系得以完全区别开 ,并用其中 1个引物对油菜杂交种的种子纯度进行了分析。结果表明 ,选择合适的引物进行RAPD分析 ,不仅是杂交品种种子纯度分析的有效手段 ,而且通过多种引物的交互使用 ,也是进行品种之间鉴别和资源保护的重要方法之一。

A simple and rapid method of trace DNA extraction on rapeseed was established. Five primers were singled out from 370 10 mer primers to distinguish 'Hybrid 59' (CMS, Brassica napus L.)and its parents obviously. Two of the primers can be used to distinguish 'Hybrid 59' from 22 other varieties and combinations. Hybrid purity of seeds can be identified with one of the primers. It was shown that RAPD with suitable primers was an effective method on hybrid seeds purification analysis, and it can be used to identify varieties and protect resources by using multiple primers alternately.