狼疮性肾炎中GSTμ基因缺失与氧化损伤的关系

Relationship Between the GSTμGene Deletion and Oxidative Injury in Lupus Nephritis

目的 :探讨狼疮性肾炎 (LN)患者谷胱甘肽转移酶 μ(GSTμ)基因缺失与氧化损伤的关系。 方法 :应用化学分析法检测 5 1例LN患者和 40例健康对照者血中一氧化氮 (NO)、脂质过氧化物 (LPO)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶 (GSH px)和谷胱甘肽 (GSH) ,用PCR法检测GSTμ基因。 结果 :LN组NO、LPO明显增高 ,SOD、GSH px、GSH明显降低。NO与LPO呈显著正相关 ,与SOD、GSH px、GSH呈显著负相关。NO、LPO与补体 3 (C3)呈显著负相关 ,与抗ds DNA、血清γ球蛋白呈显著正相关。SOD、GSH px、GSH与C3呈显著正相关 ,与抗ds DNA、血清γ球蛋白呈显著负相关。LN组GSTμ基因缺失率达 70 .5 9% ,明显高于对照组 (47.5 0 % )。GSTμ基因缺失的LN患者与GSTμ基因携带的LN患者比较 ,LPO明显增高 ,SOD、GSH px、GSH明显降低。结论 :LN患者存在氧化损伤和抗氧化能力降低 ,GSTμ基因缺失的LN患者更严重。GSTμ基因缺失可能是LN的易患因素之一。

Objective: Our purpose was to study the relationship between the glutathione S transferase class μ(GSTμ) gene and the oxidative injury in lupus nephritis(LN). Methods: Nitric oxide (NO),lipid peroxide(LPO),superoxide dismutase(SOD),glutathione peroxidase(GSH px), and glutathione(GSH) in 51 patients with LN and 40 healthy subjects were determined by using chemical analysis methods. The GSTμ gene were determined by using PCR methods. Results: The levels of NO and LPO in LN group were higher and the levels of SOD,GSH px,and GSH were lower than those of healthy group. Obvious positive correlation was observed between NO and LPO. Obvious negative correlation were observed between NO and SOD,GSH px,GSH .The obvious negative correlations were observed between NO,LPO and complement 3(C 3). Positive correlations were observed between NO,LPO and anti ds DNA,γ glubulin, and also there were positive correlations between SOD,GSH px,GSH and C 3;and negative correlations between SOD,GSH px,GSH and anti ds DNA,γ glubulin.The rate of GSTμ gene deletion in patients with LN was 70.59%,which was significantly higher than that of the controls(47.50%).The level of LPO in patients with LN without GSTμ gene was higher than that of patients with LN with GSTμ gene,and SOD,GSH px,GSH were significantly lower. Conclusion: There are oxidative injury and anti oxidation deletion in patients with LN,which of LN patients without GSTμ gene were more serious than those of LN patients with GSTμ gene. The GSTμ gene may be the marker gene of LN.This study provides theoretical and experimental basis for application of anti oxidation therapy in LN.