摘要
为了解造血干细胞动员剂G CSF和GM CSF对系统性红斑狼疮 (SLE)患者狼疮活动性的影响 ,用RT PCR法测定不同浓度G CSF和GM CSF对SLE患者外周血单个核细胞 (PBMNC)的TNF αmRNA表达的作用 ,用流式细胞术测定CD69表达和用酶联免疫吸附法测定 1 0 μg/ml的G CSF和GM CSF对PBMNC分泌IgG的影响。结果发现 :0 .1 - 2 0 μg/ml的G CSF对活动期和静止期SLE患者的PBMNC的TNF αmRNA的表达均无影响。0 1 - 1 6μg/ml或 0 1 - 2 0 μg/ml的GM CSF不影响活动期或静止期SLE患者PBMNC的TNF αmRNA的表达 ,2 μg/ml的GM CSF可增加活动期SLE患者PBMNC的TNF αmRNA的表达。 0 1 - 2 0 μg/ml的G CSF和GM CSF对静止期SLE患者PBMNC的CD69表达均无影响 ,浓度在 0 1 - 2 0 μg/ml的G CSF和 0 1 - 0 4μg/ml的GM CSF对静止期和活动期SLE患者的CD69表达无影响 ,但GM CSF的浓度达到或超过 0 8μg/ml时可增加活动期SLE患者单个核细胞CD69的表达。 1 0 μg/ml的G CSF对活动期和静止期SLE患者PBMNC的IgG分泌无明显影响 ,而相同浓度的GM CSF对活动期SLE患者PBMNC的IgG分泌有促进作用。结论 :用G CSF作自体干细胞移植的动员剂对于SLE患者可能是安全的 ,但GM CSF可增强与狼疮活动有关的三项指标 ,因此可能对活动期SLE患者产?
In order to understand the effect of hematopoietic stem cell mobilization agents, G CSF and GM CSF, on the expression of TNF α mRAN and CD69 and secretion of IgG in SLE patients′ peripheral blood mononuclear cells(PBMNC), expression of TNF α mRNA and CD69 was measured by RT PCR and flow cytometry, respectively, and IgG secretion by ELISA. The results showed that 0.1-2.0 μg/ml G CSF did not affect the expression of TNF α mRNA in active and static patients′ PBMNC treated with 0.1-2.0 μg/ml cytokines, and 2.0 μg/ml GM CSF increased the expression of TNF α mRNA in active patients′ PBMNC. G CSF and GM CSF did not interfere the expression of CD69 in active and static patients′ PBMNC, however, the expression of CD69 was significantly increased in active patients′ PBMNC treated with GM CSF at more than 8 μg/ml. There was no obvious change of IgG secretion from PBMNC induced with 10 μg/ml G CSF, while the IgG secretion was stimulated by 10 μg/ml GM CSF. It was concluded that G CSF as a mobilization agent could be safe to SLE patients, but GM CSF may cause some harmful effecs to patients because of the increase of the parameters relating to activity of lupus in active SLE patients.
出处
《中国实验血液学杂志》
CAS
CSCD
2002年第6期531-534,共4页
Journal of Experimental Hematology
基金
广东省医学科学技术研究基金项目编号A19982 0 5
