大肠阻塞模型绵羊血浆及组织中血管紧张素-Ⅱ(AⅡ)含量的变化

Changes of Plasma and Tissue AⅡ Levels in Sheep Models of Large Intenstinal Obstruction

为研究大肠阻塞动物血浆及组织中AⅡ含量的变化 ,研究以绵羊为实验动物 ,进行结肠结扎作为大肠阻塞模型动物 ,用放射免疫法对其血浆和组织中的AⅡ含量进行了测定。结果发现 :模型组绵羊动物血浆AⅡ含量在术后呈上升趋势 ,从第 2天开始显著高于手术前及对照组 (P <0 0 0 1 ) ,在术后第 2天的密集采样中低于手术前和对照组 (P <0 0 5) ,第 4天显著低于手术前和对照组 (P <0 0 1 ) ;模型组动物右心室和结肠结扎点后段AⅡ含量降低 (P<0 0 5)。结果提示 :大肠阻塞动物血浆AⅡ含量随病情的发展而迅速升高 。

The present study was designed to determine changes of plasma and tissue AⅡ concentrations in sheep models of large intestinal obstruction.The plasma and tissue AⅡ levels were measured by radioimmunoassay.Plasma AⅡ concentrations were increased significantly (P<0 001)in model group animals 2 days after operation compared with pre operation levels,this tread has lasted in the following days of the study.There were also significant differences between control and model group 2 days after operation (P<0 001).The tissue AⅡ levels were lower in the right ventricle and colon compared with control group(P<0 05).The results indicates that there is a well matched correlation between the development of the disease and increase of plasma AⅡ concentrations.