切除结膜结合淋巴组织鸡对新城疫疫苗的免疫反应Ⅰ.局部与循环抗体检测

The Effect of Immune Response of Conjunctiva-associated Lymphoid Tissue(CALT)to Eye-drop Application of the Newcastle Disease Vaccine in Chickens Ⅰ.Test of Local and Circle Antibody

实验将 60只 1日龄健康雏鸡随机分为A、B、C 3组。A组为CALT缺失组 ,B组为免疫组 ,C组为非免疫对照组。A组于 1日龄手术切除下眼睑结膜 ,A、B两组于 1 2日龄结扎鼻泪管后进行点眼免疫接种新城疫克隆 30疫苗。2 3日龄时 ,收集A、B、C各实验组鸡的血清和泪液 ,做红细胞凝集抑制试验 (HI试验 )、酶联免疫吸附试验(ELISA)。比较观察各实验组雏鸡血清和泪液抗体HI效价与IgA、IgG、IgM含量的变化特点。结果 ,1日龄手术切除下眼睑结膜后造成鸡CALT缺失 ,CALT缺失鸡点眼免疫后 ,泪液抗体HI效价与IgA、IgG、IgM含量比免疫组雏鸡低。但对雏鸡的抗体生成的规律并不产影响。故实验证实了CALT对眼区局部抗体的产生及其种类有重要的调控作用 。

Seventy five one day old male chickens were divided into equal groups A?B and C at random.Chickens in groups A were removed lower eyelid conjunctivas by surgically at one day.Chickens in groups A and B were given Newcastle Disease Clone 30 vaccine by eye drop application at eleven days old.At eighteen days old,sera of the chickens of A and B were collected for microhemagglutination inhibition(HI)test.Group C was used as control and at eighteen days old sera was collected also.At the same time of collecting sera,conjunctiva associated lymphoid tissue and Harderian glands were collected for all the chickens.Their HI titers and immune cells were observed and compared.As a result,the HI titers of group A were 2 1ong2 lower than those of vaccinated normal chickens(group B),the number of immune cells in Harderian gland reduced also.But the CALT of group B bring out earlier and grow faster than group A.Experiment results indicate that CALT is one of most important immune response tissue in ocular region,cooperating with Harderian gland,conducts local immune function.At the same time,removal lower eyelids conjunctiva surgically at one day can result losing of CALT of chickens.The chickens of losing CALT are good models for the study of mucosa immune.