摘要
利用RT_PCR方法从拟南芥 (Arabidopsisthaliana (L .)Heynh .)中克隆了生长素结合蛋白 (auxinbindingprotein 1 )cDNA ,进行了全序列测定。将该基因克隆在pBI1 2 1的 35S启动子和Nos终止子之间 ,得到植物表达载体p35EZ。通过根癌农杆菌 (Agrobacteriumtumefaciens (SmithetTownsend)Conn)介导的方法转化黄瓜 (CucumissativusL .)。对转基因植株进行了PCR和Southern检测。所得到的转基因黄瓜植株单性结实能力增强。
An auxin_binding protein 1 cDNA was isolated from Arabidopsis thaliana (L.) Heynh.by RT_PCR and confirmed by DNA sequencing. Plant expression vector p35EZ was constructed to transform the explants of Cucumis sativus L. by Agrobacterium tumefaciens (Smith et Townsend) Conn Ti plasmid_mediated method. The intergration of the transgene was identified by PCR and Southern blot analysis. It was found that the parthenocarpic ability of transgenic cucumber plants was enhanced.
出处
《植物学通报》
CSCD
北大核心
2002年第6期705-709,共5页
Chinese Bulletin of Botany
基金
重庆市科委应用基础项目 (2 0 0 1 1 1 2 5 )
