易卷曲叶表皮制片技术(NaOCl法)的改进

Improvement on NaOCl Method Used for the Liable-to-roll-up Leaf Epidermis

在利用NaOCl法制备用于光学显微镜观察的叶表皮过程中 ,一些科 /属植物的叶表皮在 1 0 0 %乙醇脱水后遇二甲苯便发生卷曲 ,增加了叶表皮制片的难度 ,甚至无法进行。本文介绍一种简便易行的方法 :在系列乙醇脱水后 ,加盖小块盖玻片 ,防止叶表皮脱水后卷曲 ,并使其保持平整 ,便于显微摄影。这种方法使得叶表皮显微制片技术更加完善。

As we prepare the leaf epidermis for examination under light microscopy using the traditional NaOCl method, sometimes difficulties occur in some materials from different families or genera in which the epidermis rolls up easily after dehydration with 100% Ethanol. This paper introduced a method by using a small piece of cover_glass to cover the epidermis before washing it with Xylene, this way the epidermis could maintain open and flat. The improvement made the technique of epidermal preparation based on NaOCl method more perfect.