摘要
用G -box家族引物对小麦光温敏核雄性不育系农大 3 3 3 8在可育与不育光温条件下进行mRNA差异显示 ,结果表明 ,在育性转换时期 ,这两种条件下的基因表达存在显著差异。回收了 12个质的差异片段并进行反Northern印迹杂交验证 ,然后对 5个阳性克隆片段HT1-G10、HT1-G3、HT2 -G2、HT1-G4和HT2 -G5进行了测序 ,同源比较显示 :HT1-G10与小麦 (Triticumaestivum)叶绿体基因rbcL和atpB的部分序列高度同源 (96% ) ;HT1-G3与小麦 (Triticumaestivum)组蛋白H2A基因高度同源 (88% ) ;另 3个片段为新基因片段。对这些基因片段的分析为揭示光温敏核雄性不育的发育机理提供了一些有效证据。
mRNA differential display with G-box family primer was used to analyze the differential expression of genes of the photoperiod-temperature sensitive genic male sterile(PTSGMS)line of wheat,BAU3338,between the sterile and fertile conditions.The result indicated that gene expression was significantly different between the two types of condition during the fertility transformation phase.The twelve qualitatively different DNA bands were identified with reverse Northern blot hybridization and five positive clones,HT1-G10,HT1-G3,HT2-G2,HT1-G4 and HT2-G5 were sequenced.The homology search indicated that HT1-G10 was highly homological (96%) to the partial sequences of Triticum aestivum chloroplast genes, rbcL and atpB ,HT1-G3 was also homological (88%) to Triticum aestivum histone H2A gene and the other three gene fragments were new sequences in GenBank.The analysis of the candidate gene fragments supplied some effective evidences to reveal the developmental mechanism of PTSGMS.
基金
国家自然科学基金杰出青年基金资助项目(30 0 2 5 0 30 )
中国科学院遗传与发育生物学研究所百人计划资助项目~~
