摘要
用SSR标记分析了 2 9份柑橘属及近缘属植物的亲缘关系。 7对SSR引物在 2 9个样品中扩增得到 114个等位基因 ,平均每个位点有 16 3个等位基因。计算匹配系数后用邻接法进行聚类 ,结果表明 ,澳洲指橘与柑橘属的亲缘关系很近 ;SSR位点的高纯合频率支持富民枳种的地位 ;枳与柑橘属的关系较远 ,枳不大可能是从柑橘属衍生而来 ;Swingle的亚属的划分以及田中的原生柑橘类和后生柑橘类的划分界线都不清晰 ;现代栽培柑橘的起源与大翼橙关系密切 ;柑橘属的枸橼、柚和宽皮橘都能很好地分离 ,支持其为现代栽培柑橘的 3个基本种的观点。
Phylogenetic relationships among 29 accessions belonging to Citrus,Poncirus,Fortunella,Microcitrus,Eremocitrus,Atlantia and Severinia were investigated using SSR markers.Seven SSR primers generated 114 polymorphic alleles,with an average of 16 3 alleles per primer.Cluster analysis via neighbour-joining method showed that Microcitrus was close to Citrus;Poncirus was distant from Citrus,which suggested that Poncirus could not be derived from Citrus.High frequency of the homozygous SSR locus supported the species status of Fumin trifoliate orange.Seperation of neither Papeda and Citrus nor Archicitrus and Metacitrus was well resolved.The present work confirmed citron,pummelo and mandarin as basic species of cultivated citrus since they could be placed into three distinct clusters.
基金
国家自然科学基金资助 (No .39970 0 81)~~
