叶绿体分裂相关基因NtFtsZ2-1在大肠杆菌中的表达与定位

CLONING OF CHLORPLAST DIVISION RELATED GENE NtFtsZ2-1 AND THE EXPRESSION IN E.COL1

FtsZ蛋白在细菌的分裂中担任着重要作用,能够在分裂位点形成一个环状结构而控制细菌的分裂过程。胞内FtsZ蛋白浓度的明显降低或异常升高均可阻断正常的细胞分裂过程进而导致丝状菌体的产生。我们为了研究烟草FtsZ蛋白与大肠杆菌FtsZ蛋白的异同,构建了烟草全长ftsZ2-1与绿色荧光蛋白EGFP的融合表达质粒并转化大肠杆菌JM109。融合表达质粒的过量表达导致宿主菌形成了丝状菌体。通过荧光显微镜观察发现NtFtsZ2-1-EGFP融合蛋白沿着宿主菌体的纵轴方向有规律地聚集成荧光点或荧光带,说明烟草FtsZ2-1蛋白能够识别宿主菌内分裂位点的定位信号并参与其细胞分裂复合物的组装。

The FtsZ protein plays a key role in bacterial cell division. It foriris a ring structure at the site of division to control the process of division. The deficiency or production of FtsZ can block the normal cell division and lead to a filamentation of the cell. We constructed a plas-tid encoding the full-length NlFtsZ2-l fused to enhanced green fluorescent protein (EGFP)al the C-terminal. Overexpression of the fusion protein in E. coli resulted in a filamentous phenotype. Fluorescence microscopy reveals the NtFtsZ2-l -KGFP fusion protein as regularly spaced dots or bands along the bacterial filaments. These results suggest that NtFtsZ2-l can recognize the signals for division site position ing in bacteria and take part in the bacterial division complexity.