我国尖音库蚊复合组蚊虫的杂交及其与Wolbachia感染的关系

Hybridization and Wolbachia infection in the Culex pipiens complex in China

为了了解我国尖音库蚊复合组蚊虫间杂交卵的不孵化现象和明确该现象与共生微生物Wolbachia感染的关系 ,对该复合组实验室种群 4个亚种进行了笼内杂交和抗生素处理后的杂交。试验表明 :在复合组蚊虫中骚扰库蚊Culexpipiensmolestus与淡色库蚊Cx.pipienspallens、致倦库蚊Cx.pipiensquinquefasciatus与尖音库蚊Cx .pipienspipiens之间存在有单向胞质不融合现象 ,骚扰库蚊的雄虫与尖音库蚊、致倦库蚊和淡色库蚊的雌虫杂交卵的孵化率分别为 0 0 6 %、 0 4 6 %和 0 19% ;该胞质不融合现象可以通过抗生素处理而消除 ,处理后骚扰库蚊雄虫与其余 3个亚种雌虫F3杂交卵的孵化率均有提高 ,分别为89 4 9% (t=3 90× 10 - 2 8<t0 0 1 =2 70 4 )、 2 3 39% (t =9 15× 10 - 7<t0 0 1 =2 6 6 0 )和 2 2 2 7% (t =5 0 8× 10 - 4<t0 0 1 =2 75 0 ) ,并可因抗生素处理而产生新的不融合类型。

In order to understand the inviability of hybrid F 1 eggs and the relationship of this to Wolbachia infection in the Culex pipiens complex in China, the crossing experiments of 4 subspecies of the complex without and with tetracycline treatment were performed. The results revealed there was unidirectional cytoplasmic incompatibility (CI) between Cx. pipiens molestus males and females of the other 3 subspecies in the complex without tetracycline treatment, and the hatching rates of eggs from matings between the males of Cx. pipiens molestus and the females of Cx. pipiens pipiens, Cx. pipiens quinquefasciatus and Cx. pipiens pallens were 0 06%,0 46% and 0 19%, respectively. Treatment with tetracycline resulted in the elimination of CI. F 3 hatching rates after tetracycline treatment between the males of Cx. pipiens molestus and the females of Cx. pipiens pipiens, Cx. pipiens quinquefasciatus and Cx. pipiens pallens were 89 49%( t=3 90×10 -28 <t 0 01 =2 704),23 39%(t=9 15×10 -7 <t 0 01 =2 660) and 22 27%( t=5 08×10 -4 <t 0 01 =2 750) , respectively. However, treatment with tetracycline resulted in the occurrence of new kinds of CI.