桃蚜不同蚜型DNA多态性的RAPD研究

RAPD analysis of different forms of the green peach aphid

采用RAPD方法 ,对全周期桃蚜的有翅产雌性母蚜、无翅性母蚜、雄蚜、卵、干母、干雌、有翅迁移蚜等蚜型的DNA遗传多态性进行了分析。结果表明 :卵的DNA多态性最大 ,性蚜次之 ,孤雌生殖蚜最小 ;卵与其它蚜型之间在遗传上具最大差异 ,其中与孤雌生殖蚜的差异大于与性蚜的 ;干母、干雌和迁移蚜之间的遗传关系最近。

The green peach aphid, Myzus persicae (Sulzer), is an important pest of agricultural and horticultural crops. Its complex life cycle and genetics are the main factors influencing population outbreaks. The RAPD technique with 7 random primers was used to analyze DNA polymorphism in seven forms of M. persicae , which were: the mother of mating forms (alate), mating females (apterous), males (alate), eggs, the fundatrix (apterous), virginoparae (apterous) and virginoparae (alate). The results showed that eggs had the largest genetic variation, followed by the sexual forms with the parthenogenetic forms having the least amount of variation. There was a close genetic relationship between the fundatrix (apterous), virginoparae (apterous) and virginoparae (alate) in the green peach aphid.