沙眼衣原体感染后大鼠输卵管糖蛋白的变化

CHANGES OF GLYCOPROTEIN IN RAT OVIDUCT AFTER CHLAMYDIAL TRACHOMATIS INFECTION

目的 研究沙眼衣原体 (chlamydialtrachomatis ,CT)感染后 ,输卵管粘膜的结构及糖蛋白的变化。方法 6 0只成年Wistar雌性大鼠随机分为实验组和对照组 ,实验组单侧卵巢囊接种沙眼衣原体E型株 ,对照组接种 2SP代替沙眼衣原体 ,分别于接种后第 0 5、 7、 14天取材 ,在光镜下观察输卵管的结构变化 ,并采用PAS Alcianblue染色显示输卵管内糖蛋白组分的变化。结果 对照组输卵管粘膜结构和糖蛋白组分无明显变化 ;实验组输卵管受损部位主要局限于粘膜层 ,PAS Alcianblue染色显示沙眼衣原体感染可导致中性糖蛋白分泌减少。结论 沙眼衣原体的感染可使输卵管糖蛋白组分发生改变 ,这可能与反复感染和宫外孕、输卵管性不孕的发生有关。

? Objective To study the alteration of glycoproteins on the mucosal surface of the oviduct after chlamydial trachomatis (CT) infection, and the correlation between the infection and the tubal infertilization or ectopic pregnancy. Method 60 Wistar female rats were randomly divided into an experimental and a control group. The rats of the experimental group were inoculated with CT, immunotype E, into one side of the ovarian bursawhile the rats of the control group were inoculated with 2SP instead. The rats of both groups were killed at day 0.5, 7, and 14 after the inoculation. The morphologic changes of the oviduct were observed by light microscopy, and the changes in thecompositionof the oviductglycoprotein were detected by PAS and Alcian blue staining. Result In the control group, oviductal mucosa and glycoprotein showed not significant changes. In the experimental group, the lesions werelimited to theoviductal mucosa. The staining result showed that CT infection decreased the secretion of the neutral glycoproteins. Conclusion CT infection can change thecompositionof the oviductglycoprotein, which might be related with repeated infections and tubal infertilization or ectopic pregnancy. 〔